2022妾㈤(y脿n)涓荤鎶€甯珮闋昏€冮粸(di菐n)妯℃摤缈�(x铆)椤屽強绛旀瑙ｆ瀽(11鏈�3鏃�)

1銆佹湁闂�(gu膩n)绱�(x矛)鑳炲洜瀛愰尟(cu貌)瑾ょ殑鏄�

A銆佹湁鍏嶇柅绱�(x矛)鑳炲強鏌愪簺鍩鸿唱(zh矛)绱�(x矛)鑳炵敘(ch菐n)鐢�

B銆佽兘鍦ㄧ窗(x矛)鑳為枔鍌抽仦淇℃伅

C銆佸叿鏈夊厤鐤(di脿o)绡€(ji茅)鍜屾晥鎳�(y墨ng)鍔熻兘

D銆佺偤铔嬬櫧璩�(zh矛)鎴栧鑲�

E銆佷竴绋窗(x矛)鑳炲彧鑳界敘(ch菐n)鐢熶竴绋窗(x矛)鑳炲洜瀛�

銆愮瓟妗堛€慐

銆愯В鏋愩€戦€氬父涓€绋窗(x矛)鑳炶兘鐢�(ch菐n)鐢熷绋窗(x矛)鑳炲洜瀛�锛孍閬搁爡(xi脿ng)閷�(cu貌)瑾ゃ€�

2銆佷笉灞簬绱�(x矛)鑳炲洜瀛愮敓鐗╁(xu茅)娲绘€х殑鏄�

A銆佸寮�(qi谩ng)鎶楁劅鏌撳拰绱�(x矛)鑳炴鍌锋晥鎳�(y墨ng)

B銆佷績閫�(j矛n)闈剁窗(x矛)鑳炵殑澧炴畺鍜屽垎鍖�

C銆佷績閫�(j矛n)鐐庣櫏閬�(gu貌)绋�锛屽奖闊跨窗(x矛)鑳炰唬璎�

D銆佷績閫�(j矛n)鎴栨姂鍒跺叾浠栫窗(x矛)鑳炲洜瀛愮殑鑶滆〃闈㈠垎瀛愮殑琛ㄩ仈(d谩)

E銆佷腑鍜屽強婧惰В鐥呮瘨浣滅敤

銆愮瓟妗堛€慐

銆愯В鏋愩€戜腑鍜屽強婧惰В鐥呮瘨鏄姉楂旂殑浣滅敤锛屼笉灞簬绱�(x矛)鑳炲洜瀛愮敓鐗╁(xu茅)娲绘€�锛孍閬搁爡(xi脿ng)閷�(cu貌)瑾ゃ€�

3銆佺敤鏂戣潵鏁疯布娉曞彲浠ュ緸浜洪珨绲勭箶娑蹭腑鐛插彇杓冪磾鐨�

A銆佸法鍣窗(x矛)鑳�

B銆丅绱�(x矛)鑳�

C銆乀绱�(x矛)鑳�

D銆佺矑绱�(x矛)鑳�

E銆佹伎绱�(x矛)鑳�

銆愮瓟妗堛€慉

銆愯В鏋愩€戜汉宸ㄥ櫖绱�(x矛)鑳炰竴鑸緸澶栧懆琛€鎴栫稉(j墨ng)鏂戣潵鏁疯布褰㈡垚鐨勭毊娉℃恫涓嵅鍙�锛屼篃鍙緸鑲烘场鐏屾礂娑叉垨鎮ｈ€呰吂鑶滈€忔瀽娑蹭腑鍒嗛洟銆�

4銆佹娓�(c猫)宸ㄥ櫖绱�(x矛)鑳炵殑鍚炲櫖鐜囧拰鍚炲櫖鎸囨暩(sh霉)甯搁噰鐢�

A銆丯BT閭勫師瑭﹂(y脿n)

B銆佹鑿岃│椹�(y脿n)

C銆佹枒铦ョ櫦(f膩)娉¤│椹�(y脿n)

D銆佽定鍖栬│椹�(y脿n)

E銆侀洖绱呯窗(x矛)鑳炲悶鍣│椹�(y脿n)

銆愮瓟妗堛€慐

銆愯В鏋愩€戝悶鍣姛鑳芥娓�(c猫)锛氬法鍣窗(x矛)鑳炲皪(du矛)椤嗙矑鎬ф姉鍘熺墿璩�(zh矛)鍏锋湁寰堝挤(qi谩ng)鐨勫悶鍣姛鑳�锛屽父鐢ㄩ洖绱呯窗(x矛)鑳�(CRBC)銆佺櫧鑹插康鐝犺弻銆侀叺姣嶇窗(x矛)鑳炵瓑浣滅偤鍚炲櫖椤嗙矑銆傚皣宸ㄥ櫖绱�(x矛)鑳炶垏闆炵磪绱�(x矛)鑳炴嚫娑插湪楂斿娣峰悎锛屾韩鑲�锛屾秱鐗�锛屾煋鑹�锛屾补閺′笅瑙€瀵�銆佽▓(j矛)鏁�(sh霉)锛岄€氶亷(gu貌)瑷�(j矛)绠楀悶鍣巼鍜屽悶鍣寚鏁�(sh霉)渚�(l谩i)鍙嶆槧宸ㄥ櫖绱�(x矛)鑳炵殑鍚炲櫖鍔熻兘锛屽苟閫氶亷(gu貌)瑙€瀵熼洖绱呯窗(x矛)鑳炵殑娑堝寲绋嬪害锛屽垽鏂峰法鍣窗(x矛)鑳炴秷鍖栧姛鑳�銆�

5銆佸鍛ㄨ涓柈鍊�(g猫)鏍哥窗(x矛)鑳炲寘鎷�

A銆佹湁鏍哥磪绱�(x矛)鑳�

B銆佹穻宸寸窗(x矛)鑳�

C銆佸柈鏍哥窗(x矛)鑳�

D銆佹穻宸寸窗(x矛)鑳炲拰鍠牳绱�(x矛)鑳�

E銆佺矑绱�(x矛)鑳�

銆愮瓟妗堛€慏

銆愯В鏋愩€戝鍛ㄨ涓柈鍊�(g猫)鏍哥窗(x矛)鑳炲寘鎷穻宸寸窗(x矛)鑳炲拰鍠牳绱�(x矛)鑳�銆�

6銆佸鍛ㄨ鍠€�(g猫)鏍哥窗(x矛)鑳炵殑姣旈噸浠嬩簬

A銆�1.001锝�1.075

B銆�1.012锝�1.030

C銆�1.030锝�1.035

D銆�1.075锝�1.090

E銆�1.092锝�1.098

銆愮瓟妗堛€慏

銆愯В鏋愩€戝鍛ㄨ涓柈鍊�(g猫)鏍哥窗(x矛)鑳�(娣嬪反绱�(x矛)鑳炲拰鍠牳绱�(x矛)鑳�)鐨勬瘮閲嶈垏绱呯窗(x矛)鑳�銆佸鏍哥櫧绱�(x矛)鑳炲強琛€灏忔澘涓嶅悓锛屼粙浜�1.075锝�1.090涔嬮枔銆�

7銆佸厤鐤祫鍖栨妧琛�(sh霉)涓祫绻旀(bi膩o)鏈殑鍙栨潗涓嶆纰虹殑鏄�

A銆佸彇鏉愬櫒姊版噳(y墨ng)閶掑埄锛岄伩鍏嶇祫绻斿彈鎿犲

B銆佸彇涓昏鐥呰畩鍗€(q奴)

C銆佸彇鐥呯伓鑸囨甯哥祫绻斿仛灏�(du矛)鐓�

D銆佸繀瑕佹檪(sh铆)鍙栭仩(yu菐n)璺濈梾鐏剁殑姝ｅ父绲勭箶鍋氬皪(du矛)鐓�

E銆佹(bi膩o)鏈伕鍙栫洝鍙兘鐨勫ぇ锛屼互姹傚彲浠ュ寘鎷洿澶氱殑绲勭箶

銆愮瓟妗堛€慐

銆愯В鏋愩€戞噳(y墨ng)鍙栨潗浜庣梾璁婄祫绻斿強鐥呰畩鑸囨甯哥祫绻斾氦鐣岃檿锛屽ぇ灏忛仼涓�銆�

8銆佸厤鐤祫鍖栨妧琛�(sh霉)绲勭箶妯�(bi膩o)鏈殑鍙栨潗涓嶅寘鎷�

A銆佹椿绲勭箶妾㈡煡妯�(bi膩o)鏈�

B銆佹墜琛�(sh霉)鍒囬櫎妯�(bi膩o)鏈�

C銆佸嫊(d貌ng)鐗╂ā鍨嬫(bi膩o)鏈�

D銆佹偅鑰呰娑�

E銆佸案楂旇В鍓栨(bi膩o)鏈�

銆愮瓟妗堛€慏

銆愯В鏋愩€戞(bi膩o)鏈殑涓昏渚�(l谩i)婧�1.娲婚珨绲勭箶锛氭噳(y墨ng)鍙栨潗浜庣梾璁婄祫绻斿強鐥呰畩鑸囨甯哥祫绻斾氦鐣岃檿锛屽ぇ灏忛仼涓�銆傛笡灏戝皪(du矛)绲勭箶妯�(bi膩o)鏈殑鎼嶅偡鑸囨摖澹�銆�2.楂旀恫銆佺┛鍒烘恫锛氬彲鐩存帴娑傜墖鎴栫稉(j墨ng)闆㈠績鍚庡彇娌夋穩鐗╂秱鐗�銆�3.鍩归(y菐ng)绱�(x矛)鑳烇細鎳告诞鍩归(y菐ng)鐨勭窗(x矛)鑳炵稉(j墨ng)闆㈠績娌夋穩鍚庡仛绱�(x矛)鑳炴秱鐗囷紝钃嬬幓鐗囦笂鐨勫柈灞ゅ煿椁�(y菐ng)绱�(x矛)鑳炵洿鎺ュ浐瀹�銆�

9銆佸厤鐤嵃璺℃硶鍗€(q奴)甯堕’鑹插父鐢ㄧ殑搴曠墿鏄�

A銆侀劙鑻簩鑳�

B銆佸洓鐢插熀鑱�(li谩n)鑻兒

C銆丄BTS

D銆佸皪(du矛)纭濆熀鑻７閰搁叝

E銆�3锛�3-浜屾皑鍩鸿伅(li谩n)鑻兒

銆愮瓟妗堛€慐

銆愯В鏋愩€戝厤鐤嵃璺℃硶鍗€(q奴)甯堕’鑹插父鐢ㄧ殑搴曠墿鏄�3锛�3-浜屾皑鍩鸿伅(li谩n)鑻兒銆�

10銆佷互涓嬫湁闂�(gu膩n)鍏嶇柅鍗拌贰娉曢尟(cu貌)瑾ょ殑瑾�(shu艒)娉曟槸

A銆侀€氶亷(gu貌)SDS-PAGE闆绘吵鍒嗛洟铔嬬櫧璩�(zh矛)妯ｅ搧

B銆佸皣铔嬬櫧璩�(zh矛)寰炲嚌鑶犺綁(zhu菐n)绉诲埌鍥虹浉鑶滀笂

C銆侀叾浣垮簳鐗╅’鑹叉娓�(c猫)鍥虹浉鑶滀笂鐨勮泲鐧借唱(zh矛)鍗€(q奴)甯�

D銆佽绋辩偤Northern blot

E銆佹湰娉曞垎杈ㄥ姏楂�锛岀壒鐣版€у拰鏁忔劅鎬т篃楂�

銆愮瓟妗堛€慏

銆愯В鏋愩€戝厤鐤嵃璺℃硶(IBT)鍙堢ūWestern-blot锛屽皣SDS-鑱氫笝鐑叞鑳哄嚌鑶犻浕娉崇殑楂樺垎杈ㄥ姏鑸囨姉鍘熸姉楂斿弽鎳�(y墨ng)鐨勯珮鐗圭暟鎬х浉绲�(ji茅)鍚�銆�

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灏忕法鎺ㄨ枽锛�2022琛�(w猫i)鐢熻硣鏍兼椹�(y脿n)涓荤鎶€甯ā鎿反缈�(x铆)椤屽強绛旀瑙ｆ瀽

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